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研究表明,活化巨噬细胞是对曼氏血吸虫产生保护免疫的主要效应细胞,但其作用机理尚未明了。TNF是一种介导巨噬细胞杀伤肿瘤细胞的非氧化性效应因子,从活化巨噬细胞的培液中可检测到与TNF相似的细胞毒素的反应性。为此作者研究了TNF在巨噬细胞介导的杀血吸虫童虫中的作用。细胞毒血清或肿瘤坏死血清(TNS)的制备,采用C_3H/Hem小鼠静脉注射2×10~6克隆形成单位BCG,或经皮肤接种35条曼氏血吸虫尾蚴,BCG感染后2周,血吸虫感染后8～10周,再静脉注入10μg大肠杆菌脂多糖(LPS),2h后放血分离血清即为TNS。取未经处理的小鼠或只接受过感染的或只注射
Studies have shown that activated macrophages are the main effector cells that produce protective immunity against Schistosoma mansoni, but the mechanism of action is unclear. TNF is a non-oxidative effector that mediates macrophage killing of tumor cells. Cytotoxic reactivity similar to that of TNF is detectable in the culture of activated macrophages. To this end the authors investigated the role of TNF in macrophage-mediated Schistosoma japonicum. Cytotoxic serum or tumor necrosis serum (TNS) was prepared by intravenous injection of 2 × 10 ~ 6 clonogenic BCG in C_3H / Hem mice or 35 inoculation of cercariae of Schistosoma mansoni, 2 weeks after BCG infection, schistosomiasis infection After 8 to 10 weeks, 10μg of E. coli lipopolysaccharide (LPS) was intravenously injected again, and the blood was separated 2 hours later to separate the serum into TNS. Take untreated mice or only infected or injected only