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实验选用多克隆的犬抗体作为原发反应剂(primary reagent),验证多克隆抗体能否代替单克隆抗体作为原发反应剂。犬抗体(AB1)是用婴儿利什曼原虫前鞭毛体(LiF2)的94-67kDa组分免疫后获得,在体内外均能使大型利什曼原虫失去感染力。给BALB/c小鼠LiF2制剂和胞壁酰二肽(MDP)
Polyclonal dog antibody was selected as the primary reagent to verify whether the polyclonal antibody can replace the monoclonal antibody as the primary reagent. Canine antibodies (AB1) were obtained after immunization with the 94-67 kDa fraction of Leishmania infantum (LiF2), which infect large Leishmania in vitro and in vivo. BALB / c mice LiF2 formulation and muramyl dipeptide (MDP)