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最近在印度和孟加拉国出现一种不明来历的新型霍乱弧菌血清型O 139,引起了远东地区霍乱的大规模蔓延。经脉冲凝胶电泳(PFGE)及锚定引物多聚酶链反应(AP-PCR)分析霍乱弧菌DNA的多态性,从而对这一新流行菌株与引起第7次霍乱流行的菌株O1型之间的遗传学关系做了大量研究。 O139分离菌可产生相当数量的霍乱毒素——一种能引起腹泻的重要毒性因子,由染色体上ctxA、ctxB两基因编码(此两种基因常常前后排列)。这些分离菌均表达TcPA
The recent outbreak of an unknown Vibrio cholera serogroup O 139 in India and Bangladesh has caused a massive spread of cholera in the Far East. Polymorphism of Vibrio cholerae DNA was analyzed by pulse gel electrophoresis (PFGE) and anchored primer polymerase chain reaction (AP-PCR), and thus between the new epidemic strain and the strain O1 causing the seventh cholera epidemic The genetic relationship has done a lot of research. O139 isolates can produce a significant amount of cholera toxin - an important toxic factor that causes diarrhea - encoded by the two genes ctxA and ctxB on chromosomes (these two genes often precede and next). These isolates express TcPA