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在渗透胁迫下,测定了不同抗旱性小麦(抗旱性强的品种洛旱6号和抗旱性弱的品种周麦18)幼苗氨同化酶及相关参数的变化.结果表明:小麦生物量在渗透胁迫下明显降低,且抗旱性弱的周麦18降幅较大.铵态氮含量随胁迫程度的增加而增加,且周麦18增加较明显;谷氨酰胺合成酶(GS)活性在不同抗旱性品种间表现不同,抗旱性强的洛旱6号在低渗透胁迫下显著增加,在高渗透胁迫下明显降低,而周麦18随胁迫程度的增加逐渐降低;依赖还原型辅酶Ⅰ的谷氨酸脱氢酶(NADH-GDH)活性随胁迫程度的增加逐渐加大,低渗透胁迫下周麦18增加较明显,高渗透胁迫下洛旱6号增幅较大;依赖氧化型辅酶Ⅰ的谷氨酸脱氢酶(NAD+-GDH)和依赖氧化型辅酶Ⅱ的异柠檬酸脱氢酶(NADP-ICDH)活性均随胁迫程度的增加而增加,周麦18的NAD+-GDH活性、洛旱6号的NADP-ICDH活性增幅较大.表明小麦抗旱性的提高与铵态氮同化的增强有关,低渗透和高渗透胁迫下分别依赖GS和NADH-GDH活性的增加.
Under osmotic stress, the changes of ammonia assimilation enzymes and related parameters of seedlings of different drought-tolerant wheat varieties (drought-resistant cultivar Luohan 6 and drought-resistant cultivar Zhoumai 18) were measured.The results showed that under the osmotic stress , And decreased significantly in Zhoumai 18 with weak drought resistance.The content of ammonium nitrogen increased with the increase of stress degree and the increase of Zhoumai 18 increased obviously.The activity of glutamine synthetase (GS) The drought resistance of Drought drought 6 increased significantly under low osmotic stress and decreased significantly under high osmotic stress, while that of ZHOU Mai 18 decreased gradually with the increase of stress. The activity of glutamic acid dependent on reduced coenzyme Ⅰ The activity of NADH-GDH gradually increased with the increase of stress level. Under the condition of low osmotic stress, the increment of NADH-GDH was more obvious. Under drought stress, The activities of NAD + -GDH and NADP-ICDH were both increased with the increasing of stress degree. NAD + -GDH activity of Zhoumai 18, NADP of Luohan 6 -ICDH activity increased significantly, indicating that the improvement of wheat drought resistance and ammonium nitrogen For reinforcement, respectively, dependent increase GS and NADH-GDH activity and low permeability under high osmotic stress.