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目的:探讨早期运动训练能否通过增强脑线粒体呼吸功能预防MPTP对中脑多巴胺能神经元的损伤。方法:32只雄性小鼠(8~9周龄)随机分为安静组、运动组。运动组连续5周,每天一次中等强度(12米/分,20分钟)跑台训练。然后以上两组再各自随机分成两组,分别注射生理盐水或中等剂量MPTP(30mg/kg×2次),最后分为安静+生理盐水、运动+生理盐水、安静+MPTP、运动+MPTP四组,每组8只。各组分别于药物注射后第二周进行脑线粒体能量测试,免疫组织化学方法观察中脑黑质酪氨酸氢化酶(TH)阳性细胞数量。结果:运动+MPTP组脑线粒体态3呼吸速率显著高于安静+MPTP组(P<0.01),运动+盐水组和安静+盐水组脑线粒态3呼吸速率无显著差异(P>0.05),运动+MPTP组TH阳性细胞数显著多于安静+MPTP组(P<0.01)。结论:早期运动训练能通过增强脑细胞线粒体呼吸功能,减少MPTP对中脑多巴胺能神经元损伤。
AIM: To investigate whether early exercise training can prevent MPTP from damaging the midbrain dopaminergic neurons by enhancing mitochondrial respiratory function. Methods: Thirty-two male mice (8-9 weeks old) were randomly divided into quiet group and exercise group. Exercise group for 5 weeks, once a day medium-intensity (12 m / min, 20 minutes) treadmill training. The above two groups were then randomly divided into two groups: normal saline or moderate dose of MPTP (30mg / kg × 2 times), and finally divided into quiet + saline, exercise + saline, quiet + MPTP, , Each group of 8. The brain mitochondrial energy was measured in the second week after drug injection, and the number of tyrosine hydrogenase (TH) positive cells in midbrain was observed by immunohistochemistry. Results: The respiration rate of brain mitochondrial state 3 in exercise + MPTP group was significantly higher than that in quiet + MPTP group (P <0.01), but there was no significant difference between exercise + saline group and quiet + saline group (P> 0.05) The number of TH positive cells in the MPTP group was significantly more than that in the quiet + MPTP group (P <0.01). Conclusion: Early exercise training can reduce the damage of dopaminergic neurons in midbrain by increasing the respiratory function of mitochondria in brain cells.