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应用划痕标记染料示踪技术检测β－胡萝卜素对促癌剂ＴＰＡ抑制ＮＩＨ／３Ｔ３细胞间隙连接通讯（ＧＪＩＣ）功能的影响，同时对细胞膜流动性和细胞膜唾液酸含量的变化与ＴＰＡ抑制细胞ＧＪＩＣ功能的关系，以及β－胡萝卜素对这些变化的作用进行了研究。结果显示：β－胡萝卜素对ＮＩＨ／３Ｔ３细胞ＧＪＩＣ功能有促进作用，并对ＴＰＡ抑制ＮＩＨ／３Ｔ３细胞ＧＪＩＣ功能有一定的拮抗作用，随剂量升高上述作用表现为增强趋势；ＴＰＡ抑制细胞ＧＪＩＣ功能的同时显著升高膜流动性和膜唾液酸含量，而β－胡萝卜素对ＴＰＡ诱导的这些效应有拮抗作用。
Scratch Marker Dye Tracer Technique Detects the Effect of β-carotene on the Inhibitory Effect of Promoter TPA on NIH/3T3 Cell Gap Junction Communication (GJIC) Function, Simultaneously Changes in Cell Membrane Fluidity and Cell Membrane Sialic Acid Content and TPA Inhibitory Cells GJIC The relationship between function and the effect of β-carotene on these changes were studied. The results showed that β-carotene promoted the GJIC function of NIH/3T3 cells and inhibited the GJIC function of NIH/3T3 cells by TPA. The above effects were enhanced with increasing doses; TPA inhibited GJIC function of cells. While significantly increasing membrane fluidity and membrane sialic acid content, β-carotene has antagonistic effects on these effects induced by TPA.