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正确评估卵母细胞的质量对于改善人类辅助生殖技术的治疗结局有重要意义。卵母细胞的评估方法有多种,其中,颗粒细胞和卵泡液因子在评估卵母细胞质量中的作用备受关注。研究显示颗粒细胞的凋亡率与卵母细胞的发育能力呈负相关;卵泡液中的雌激素水平或雌激素与雄激素(或孕激素)的比率、胰岛素样生长因子(IGF)和胰岛素样生长因子结合蛋白(IGFBP)的表达与卵母细胞的质量有关,而卵泡液中的其他因子,如瘦素、抗苗勒激素等与卵母细胞质量之间的关系尚不明确。卵丘细胞的基因表达、卵泡液因子及代谢组学的进一步研究,有望成为评估卵母细胞质量的重要工具。
Correct assessment of the quality of the oocyte is of great importance to improving the therapeutic outcome of human assisted reproductive technology. There are many ways to evaluate oocytes, among which, the role of granulosa cells and follicular fluid factors in evaluating oocyte quality is of great concern. Studies have shown that the apoptotic rate of granulosa cells is negatively correlated with the developmental capacity of oocytes; the level of estrogen in follicular fluid or the ratio of estrogen to androgens (or progesterone), insulin-like growth factor (IGF) and insulin-like The expression of growth factor binding protein (IGFBP) is related to the quality of oocytes. The relationship between other factors in follicular fluid such as leptin, anti-Müllerian hormone and oocyte quality is unclear. Further study of cumulus cell gene expression, follicular fluid factor and metabonomics is expected to be an important tool to evaluate oocyte quality.