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里那醇可诱导植物产生防御物质从而阻止昆虫及病原菌的伤害。本文以拟南芥植株为材料,研究了里那醇对小菜蛾取食选择性的影响,测定了里那醇处理下小菜蛾生长量及存活率。采用非损伤微测技术分别检测了里那醇熏蒸及未熏蒸拟南芥对小菜蛾口腔分泌物及里那醇瞬时处理的植株叶片跨膜Ca~(2+)和H~+流的变化。结果表明,小菜蛾幼虫对里那醇熏蒸处理的植株选择性较差,用里那醇熏蒸后的拟南芥植株喂养小菜蛾幼虫,幼虫的生长量及存活率均低于对照组。小菜蛾口腔分泌物和里那醇熏蒸处理均导致拟南芥叶组织跨膜Ca~(2+)和H~+离子迅速外流,且小菜蛾口腔分泌物的作用更大。而对照中Ca~(2+)和H~+离子流整体均无明显变化。研究结果证明里那醇作为防御信号参与了拟南芥对昆虫的防御反应,叶组织跨膜Ca~(2+)和H~+离子流变化可能是拟南芥识别昆虫口腔分泌物与里那醇两种不同刺激的初始信号。
Rinalcohol induces plants to produce defensive substances that prevent the damage of insects and pathogens. In this paper, Arabidopsis plants as materials, to study the selectivity of latina alcohol feeding on Plutella xylostella, measured the growth and survival of the diamondback moth larvae. Non-invasive micro-techniques were used to detect the transmembrane Ca 2+ and H + currents in leaves of instantaneous-treated oral amphotericin (Plutella xylostella) and rinolol-treated florentin and non-fumigated Arabidopsis thaliana respectively. The results showed that the larvae of Plutella xylostella larvae were less selective to rinacol fumigation. The larvae of Plutella xylostella fed with Arabidopsis thaliana fumigated with genital alcohol had lower growth and survival rates than the control. Both oral secretions and rinalinal fumigation of Plutella xylostella caused rapid exocytosis of transmembrane Ca 2+ and H + ions in Arabidopsis thaliana leaf tissues, and oral insecticidal activity of Plutella xylostella was greater. However, there was no obvious change in the whole Ca ~ (2+) and H ~ + ion currents in the control. The results show that rileolol as a defense signal involved in Arabidopsis defense responses to insects, leaf tissue transmembrane Ca ~ (2 +) and H ~ ion flux changes may be Arabidopsis recognition insect oral secretions and that Alcohol two different stimuli of the initial signal.