论文部分内容阅读
背景:脑醒喷鼻剂由川芎和石菖蒲等中药组成,《神农本草经》谓其“主脑卒中入脑”之功效。目的:观察脑醒喷鼻剂对大鼠局灶性脑缺血再灌注损伤脑组织自由基及一氧化氮合酶变化的影响,并与经典西药尼莫地平相比较。设计:随机对照实验。单位:一所中医药大学医院的内科。对象:清洁级成年雄性Wistar大鼠70只,随机分为7组:脑醒喷鼻剂高剂量组(高剂组),脑醒喷鼻剂中剂量组(中剂组),脑醒喷鼻剂低剂量组(低剂组),尼莫地平腹腔注射组(尼莫地平组),生理盐水喷鼻剂组(生理盐水组),溶酶喷鼻剂组(溶酶组),假手术组,每组10只。方法:除假手术组外,其他6组阻断大鼠左侧大脑中动脉建立大鼠局灶脑缺血再灌注模型。在造模前3d及再灌注期间,脑醒喷鼻剂高、中、低剂组分别以含川芎嗪和石菖蒲生药5.4,2.7,1.08mg/(kg·d)和1.35,0.54,0.27g/(kg·d)的剂量滴鼻,3次/d;生理盐水组和溶酶组以生理盐水、溶酶喷鼻剂0.18mL/(kg·d)滴鼻,3次/d;尼莫地平组用尼莫地平注射液0.8mg/(kg·d)腹腔注射,2次/d;假手术组按常规饲养至实验取材。用比色法检测丙二醛、超氧化物歧化酶及一氧化氮合酶。主要观察指标:①不同剂量脑醒喷鼻剂及其他组动物脑组织丙二醛、超氧化物歧化酶及一氧化氮合酶活性变化。②将不同剂量脑醒喷鼻剂组与尼莫地平组做比较。结果:造模时死亡8只动物,进入结果分析62只。①丙二醛含量:高剂组、尼莫地平组明显低于生理盐水组[(0.92±0.32),(0.87±0.39),(1.35±0.34)μmol/g,P<0.05],但高剂组和尼莫地平组间无差异。②超氧化物歧化酶活性:高剂组、尼莫地平组明显高于生理盐水组[(35.64±11.67),(33.88±7.15),(20.70±3.88)NU/mg,P<0.05],但高剂组和尼莫地平组间无差异。③一氧化氮合酶活性及超氧化物歧化酶活性:高剂组、尼莫地平组明显高于生理盐水组[(4.64±1.22),(5.00±1.10),(3.08±1.12)mkat/g,P<0.05],但高剂组和尼莫地平组间无差异。④中、低剂组和溶酶组超氧化物歧化酶及一氧化氮合酶活性有所升高,丙二醛含量有所降低,但与生理盐水组比较无差异。结论:脑醒喷鼻剂高剂量组能防止脑缺血缺氧所致的脂质过氧化,使丙二醛生成减少,清除自由基损害,并增加一氧化氮合酶活性,对脑组织的缺血再灌注损伤有保护作用,与尼莫地平的作用相当。
Background: Naoxing Nasal Spray is composed of traditional Chinese medicine such as Chuanxiong and Shichangpu. The Shennong Bencaojing said that it has the effect of “main brain stroke into the brain.” OBJECTIVE: To observe the effects of Naoxing Nasal Spray on the changes of free radicals and nitric oxide synthase in the brain of rats with focal cerebral ischemia-reperfusion injury, and compare it with the classical western drug Nimodipine. Design: Randomized controlled trials. Unit: Internal medicine of a Chinese medicine university hospital. PARTICIPANTS: Seventy adult male Wistar rats of clean grade were randomly divided into 7 groups: Naoxing nasal spray high dose group (high dose group), Naoxing nasal spray middle dose group (neutral dose group), awake nasal spray Low dose group (low dose group), nimodipine group (nimodipine group), saline nasal group (saline group), lyase nasal group (lysozyme group), sham group , 10 in each group. Methods: In addition to the sham operation group, the other 6 groups were used to block the left middle cerebral artery to establish a rat model of focal cerebral ischemia-reperfusion. During the 3 days before modeling and reperfusion, Naoxing nasal spray high, middle and low dose groups were respectively containing Ligustrazine and Shichangpu crude drug 5.4,2.7,1.08mg/(kg·d) and 1.35,0.54,0.27g / (kg · d) dose of nasal drops, 3 times / d; saline group and lysozyme group with saline, lyase nasal spray 0.18mL / (kg · d) nasal drops, 3 times / d; Nemo Diping group was injected intraperitoneally with nimodipine injection 0.8 mg/(kg·d) twice a day; the sham operation group was raised by routine feeding to the experiment. Malondialdehyde, superoxide dismutase and nitric oxide synthase were detected by colorimetry. MAIN OUTCOME MEASURES: Changes in the activity of malondialdehyde, superoxide dismutase, and nitric oxide synthase in cerebral tissues of different doses of Naoxing Nasal Spray and other groups. 2 Compare different doses of Naoxing Nasal Spray with Nimodipine. RESULTS: Eight animals died at the time of modeling, and 62 results were analyzed. 1 Malondialdehyde content: The high-dose group and nimodipine group were significantly lower than the saline group [(0.92±0.32), (0.87±0.39), (1.35±0.34) μmol/g, P<0.05], but high dose There was no difference between the group and the nimodipine group. 2Superoxide dismutase activity: The high-dose group and the nimodipine group were significantly higher than the saline group [(35.64±11.67), (33.88±7.15), (20.70±3.88) NU/mg, P<0.05], but There was no difference between the high-dose group and the nimodipine group. 3Nitric oxide synthase activity and superoxide dismutase activity: The high-dose group and nimodipine group were significantly higher than the saline group [(4.64±1.22), (5.00±1.10), (3.08±1.12) mkat/g (P < 0.05), but there was no difference between the high-dose group and the nimodipine group. 4 The activities of superoxide dismutase and nitric oxide synthase in the medium and low-dose group and lysozyme group were increased and the content of malondialdehyde was decreased, but there was no difference between the group and the saline group. Conclusion: Naoxing Nasal Spray high-dose group can prevent lipid peroxidation caused by cerebral ischemia and hypoxia, reduce the production of malondialdehyde, scavenge free radical damage, and increase the activity of nitric oxide synthase in brain tissue. Ischemia-reperfusion injury has a protective effect and is equivalent to that of nimodipine.