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在急性、慢性在体大鼠及离体脑片癫疒间 模型上观察 EEG、深部电图、神经元网络及单个神经元的电活动 ,研究中枢同步化电振荡行为在癫疒间发生中的重要作用。实验结果表明 :离体脑片 ( n=40 )上强直电刺激海马 Schaeffer侧枝 ,32 .5% CA1神经元全细胞记录呈现 3～ 1 0 0 Hz膜电位振荡 ,这种电振荡能促进细胞外场电位癫疒间 活动形式的转化。急性癫疒间 模型上 ( n=1 0 ,in vivo) ,反复强直电刺激海马或中部颞叶新皮质可诱发海马区 4～ 7Hz和中部颞叶新皮质 2 0 Hz( n=1 1 )深部电图电振荡。慢性强直电刺激大鼠背侧海马 ( n=1 2 )或中部颞叶新皮质 ( n=1 1 ) ,7～ 8d后出现这些脑区和 EEG电振荡活动的频率及幅度的增加。巨大 EEG节律性电振荡活动成为电图和癫疒间 行为性发作的先兆。说明过度激活海马 -内嗅皮质 -颞叶新皮质产生的同步化电振荡可能是颞叶癫疒间发生的重要原因之一。
The electrical activity of EEG, deep electrograms, neuronal networks and single neurons were observed in acute and chronic rat epilepsy model and in vitro. The effects of centrally synchronized electrical oscillatory behavior on the development of epilepsy Important role. The experimental results showed that the whole brain cell recording of 32.5% CA1 neurons showed 3 ~ 100 Hz membrane potential oscillations during the stimulation of hippocampal Schaeffer collaterals on the isolated brain slices (n = 40), which could promote the extracellular field Potential changes in the form of activity between epilepsy. In the acute epilepsy model (n = 10, in vivo), repeated tetanic stimulation of the hippocampus or middle temporal cortex neocortex induced deep hippocampal 4 ~ 7Hz and central temporal lobe neocortex 20 Hz (n = 11) Electrical oscillation. Chronic tetanic stimulation of the dorsal hippocampus (n = 12) or middle temporal lobe neocortex (n = 11) in rats resulted in an increase in frequency and amplitude of electrical oscillations in these brain regions and EEGs after 7-8 days. Huge EEG rhythmic electrical activity becomes a harbinger of behavioral seizures between electrograms and epilepsy. These results suggest that synchronous activation of the hippocampus-entorhinal cortex-temporal cortical neocortex may be one of the important causes of temporal lobe epilepsy.