目的 :观察左旋多巴和DA对中脑原代培养细胞的毒性作用。 方法 :采用大鼠胚胎中脑原代细胞培养法 ,运用TH免疫荧光染色和 [3H]DA摄取率检测DA能神经元的存活数和功能 ;GFAP免疫荧光染色检测星形胶质细胞的存活数 ;以及MTT检测非DA能神经元的存活数。 结果 :左旋多巴或DA处理后的TH阳性和GFAP阳性细胞数以及细胞存活率均显著低于加药前基数 ,且呈剂量依赖性 ;同时残存细胞体积变小 ,突起减少、变短或断裂。TH阳性细胞和GFAP阳性细胞比非DA能神经元更易受损。结论 :左旋多巴和DA对中脑原代细胞培养中的DA能神经元和非DA能神经元均有毒性作用 Objective: To observe the toxic effects of levodopa and DA on primary cultured cells of midbrain. Methods: Rat embryonic mesencephalic cell culture was used to detect the viability and function of DA neurons by TH immunofluorescence staining and [3H] DA uptake rate. GFAP immunofluorescence staining was used to detect the viability of astrocytes ; And MTT to detect the number of non-DA neurons survival. Results: The numbers of TH positive cells, GFAP positive cells and cell viability after L-dopa or DA treatment were significantly lower than those before dosing, and in a dose-dependent manner. At the same time, the remnant cells became smaller and the protrusions decreased, shortened or ruptured . TH-positive cells and GFAP-positive cells are more vulnerable than non-DA neurons. CONCLUSION: Both levodopa and DA have toxic effects on DA neurons and non-DA neurons in midbrain primary cell culture