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目的建立HPLC法同时测定连翘中连翘苷和连翘酯苷A的含量。方法在色谱柱Agilent Zobax SB C18(4.6 mm×250 mm,5μm)上进行分离,柱温为30℃,流动相:A相为乙腈;B相为0.2%甲酸水溶液,梯度洗脱:0~28 min,15%A:85%B,28~33 min,15%→20%A:85%→80%B,33~38 min,20%A:80%B,38~50 min,20%→25%A:80%→75%B,50~60 min,25%A:75%B,流速:1.0 ml·min-1,检测波长:278 nm。结果样品中2种成分得到良好分离,连翘苷和连翘酯苷A分别在0.01975~0.05925 mg·ml-1(r=0.9997),和0.0998~0.2994 mg·ml-1(r=0.9996)范围内进样量与峰面积呈良好的线性关系,回收率分别为99.49%和99.18%,RSD分别1.19%和1.04%,样品溶液12 h内稳定。结论该方法操作简单,结果准确,专属性强,可用于连翘药材的质量控制。
Objective To establish a HPLC method for the simultaneous determination of forsythin and forsythiaside A in Forsythia suspensa. Methods The separation was carried out on an Agilent Zobax SB C18 column (4.6 mm × 250 mm, 5 μm) with a column temperature of 30 ° C. The mobile phase consisted of acetonitrile in phase A and 0.2% formic acid in phase B, gradient elution from 0 to 28 min 15% A: 85% B 28-33 min 15% → 20% A: 85% → 80% B 33-38 min 20% A: 80% B 38-50 min 20% → 25% A: 80% → 75% B, 50-60 min, 25% A: 75% B, flow rate: 1.0 ml · min-1, detection wavelength: 278 nm. Results The two components in the sample were well separated. The contents of forsythin and forsythoside A were in the ranges of 0.01975 ~ 0.05925 mg · ml -1 (r = 0.9997) and 0.0998 ~ 0.2994 mg · ml -1 (r = 0.9996) The internal injection volume and the peak area showed a good linear relationship, the recovery rates were 99.49% and 99.18%, RSD 1.19% and 1.04% respectively, and the sample solution was stable within 12 h. Conclusion The method is simple, accurate and specific, and can be used for the quality control of Forsythia suspensa.