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目的建立海马巴戟胶囊中人参皂苷Rg1、Re、Rb1的含量测定方法,为该药质量标准研究和完善提供依据。方法采用HPLC法,色谱柱:ZORBAX SB-C18柱(4.6 mm×250 mm,5μm)。流动相:乙腈-水(梯度洗脱),柱温:35℃,检测波长为203 nm,流速:1.0 ml·min-1。结果人参皂苷Rg1、Re、Rb1浓度分别在0.0540~0.8640、0.0616~0.9860、0.1258~2.012 mg·ml-1范围内具有良好线性关系,平均加样回收率分别为99.12%、99.60%、98.47%。结论结果表明该方法准确可靠,重复性好,结果稳定。
Objective To establish a method for the determination of ginsenoside Rg1, Re, Rb1 in the hippocampus of Juba, providing basis for the study and improvement of the quality standard of this medicine. Methods The HPLC method was adopted. Column: ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase was acetonitrile-water (gradient elution). The column temperature was 35 ℃, the detection wavelength was 203 nm and the flow rate was 1.0 ml · min-1. Results The concentrations of ginsenosides Rg1, Re and Rb1 were linear in the range of 0.0540 ~ 0.8640,0.0616 ~ 0.9860 and 0.1258 ~ 2.012 mg · ml-1, respectively. The average recoveries were 99.12%, 99.60% and 98.47%, respectively. Conclusion The results show that the method is accurate and reliable, good repeatability and stable results.