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目的通过确定患儿血清中存在的不规则抗体的性质及效价,配合型输血,以评估其输血疗效。方法应用试管法检测ABO及RhD血型,使用微柱凝胶法、凝聚胺、抗球蛋白法对患儿血清及放散液中的不规则抗体进行筛查鉴定,并测定血清中抗体的效价。结果患儿血型为“O”CcDEe,血清及放散液中存在类同种抗-D,其血清中抗体效价为8。随着给予患儿输注RhD阴性红细胞输注剂量与次数增加,患儿血红蛋白值逐渐增高,类同种抗-D效价逐渐减低。结论应根据类同种自身抗体特异性,选择输注其对应抗原阴性红细胞,才能确保临床输血安全性与有效性。
Objective To determine the transfusion efficacy by determining the nature and titer of the irregular antibodies present in the serum of children and coordinating transfusion. Methods Blood samples of ABO and RhD were detected by in vitro test, and the antibody in blood serum and the irregular antibody in the effusion were screened by microcolumn gel method, polybrene and antiglobulin method, and the antibody titer in serum was determined. Results The blood type of children was “O” CcDEe. The same kind of anti-D existed in serum and effusion, and the antibody titer in serum was 8. With the infusion of RhD-negative erythrocytes given to infants increased the number and frequency of transfusions, children with hemoglobin value gradually increased, similar anti-D titer decreased gradually. Conclusion According to the same type of autoantibodies specificity, the choice of infusion of its corresponding antigen-negative red blood cells in order to ensure the safety and effectiveness of clinical transfusion.