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花刺参体壁经碱解、不同浓度的酒精沉淀氯化脱色及乙酸钾纯化,分别得花刺参酸性粘多糖SⅥ及SⅦ。2种多糖均显示电泳纯,超离心分析SⅥ S_(20w)=3.09,SⅦ S_(20w)=2.12;与碱性染料天青工异染反应,SⅥ和SⅦ的异染峰分别位移于514nm及507nm;SⅥ分子是由氨基半乳糖、葡萄糖醛酸、岩藻糖及硫酸基组成,其分子比值为1:1.21:1.29:4.62;SⅦ分子是由岩藻糖及硫酸基组成,其分子比值为1:0.99。而刺参粘多糖(Sjamp)的超离心分析S 20w=2.12。与碱性染料天青Ⅰ异染峰为515nm,Sjamp分子也是由氨基半乳糖,葡萄糖醛酸、岩藻糖及硫酸基组成,其分子比值为1:1.11:1.20:487。
Saccharomyces cerevisiae was alkali-lysed, decolorized with different concentrations of alcohol, decolorized by chlorination, and purified with potassium acetate to obtain acid sticky polysaccharide SVI and SVII of Stichopus japonicus. The two polysaccharides were all electrophoretically pure. The ultracentrifugation analysis showed that SVI S 20w = 3.09 and SVII S 20w = 2.12. When reacted with the basic dye Astrotech, the peaks of the discoloration of SVI and SVII were shifted to 514 nm. 507nm; SVI molecule is composed of galactose, glucuronic acid, fucose and sulfate groups, and its molecular ratio is 1:1.21:1.29:4.62; SVII molecule is composed of fucose and sulfate groups, and its molecular ratio is 1:0.99. The ultracentrifugal analysis of Sjamp mucopolysaccharide (Sjamp) S 20w=2.12. The peak of isochromaticity with the basic dye azure I is 515 nm, and the Sjamp molecule is also composed of galactose, glucuronic acid, fucose, and sulfate groups, and its molecular ratio is 1:1.11:1.20:487.