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目的:观察丹参总酚酸(TSA)对离体大鼠心肌缺血再灌注(I/R)心脏的保护作用。方法:采用Langendorff离体心脏恒压灌流模型,大鼠分为空白对照组、模型组、停灌前给药组、复灌给药组。停灌前给药组为正常灌流平衡20 min后,分别用质量浓度0.2,0.1 g.L-1的TSA灌流液灌注10 min,停灌31 min,复灌30 min,用于观察TSA对再灌后心律失常的影响;复灌给药组正常灌流平衡30 min,停灌31 min,复灌分别给予质量浓度0.2,0.1 g.L-1 TSA灌流液15 min,再用正常灌流液灌注15min,用于观察TSA对心功能的影响。结果:停灌前给药,TSA能明显减少I/R后大鼠心律失常发生次数和持续时间(P<0.05～0.01);复灌后给药,与模型相比TSA高剂量组可显著减轻缺血再灌注诱导的冠脉流量,左室发展压(LVDP),左室内压最大变化速率(±dp/dtmax)下降,左室舒张末压(LVEDP)的升高(P<0.05～0.01),低剂量组仅冠脉流量,+dp/dtmax的恢复高于模型组(P<0.05)。结论:TSA能减轻离体心脏缺血再灌注诱导的冠脉损伤,促进复灌心肌功能恢复,具有明显保护作用。
Objective: To observe the protective effect of salvianolate (TSA) on isolated rat hearts from myocardial ischemia reperfusion (I / R). Methods: Langendorff isolated heart constant pressure perfusion model was used. The rats were divided into blank control group, model group, pre-stop irrigation group and reperfusion administration group. Before stopping irrigation, the rats in the treatment group were given 20% of normal perfusion for 20 min, then perfused for 10 min with TSA perfusate of 0.2 and 0.1 gL-1, respectively, for 31 min and reperfusion for 30 min, The normal perfusion of reperfusion administration group was balanced for 30 min and stopped for 31 min. Reperfusion perfusion was performed with TSA perfusate of 0.2 and 0.1 gL-1 for 15 min respectively and then perfusion with normal perfusate for 15 min Effect of TSA on Cardiac Function. Results: Pretreatment with TSA significantly reduced the number and duration of arrhythmia in rats after I / R (P <0.05 ~ 0.01). After reperfusion administration, TSA high dose group significantly reduced Coronary artery flow, LVDP, ± dp / dtmax and LVEDP increased (P <0.05 ~ 0.01) after ischemia / reperfusion. The coronary flow and + dp / dtmax in low dose group were higher than those in model group (P <0.05). Conclusion: TSA can relieve the coronary artery injury induced by ischemia-reperfusion in vitro and promote the recovery of myocardial function after reperfusion, which has a significant protective effect.