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目的对短葶山麦冬进行遗传多样性分析。方法利用SRAP分子标记技术对47份短葶山麦冬材料进行遗传多样性分析。结果 15对引物共扩增出323条多态性带,平均多态位点百分率为88.47%。平均引物多态信息量(PIC)为0.90。Nei’s基因多样性指数(H)为0.197 7,Shannon’s信息指数(I)为0.319 0。遗传分化系数(Gst)为0.252 8。UPGMA聚类表明遗传相似系数为0.59～0.99。结论短葶山麦冬遗传多样性水平较高,遗传变异主要在居群内。
Objective To analyze the genetic diversity of Ophiopogon japonicus. Methods The genetic diversity of 47 Ophiopogon japonicus Ophiopogon japonicus was analyzed using SRAP molecular markers. Results A total of 323 polymorphic bands were amplified with 15 pairs of primers. The average percentage of polymorphic loci was 88.47%. The average primer polymorphism information (PIC) was 0.90. Nei’s gene diversity index (H) was 0.197 7, Shannon’s information index (I) was 0.3190. The genetic differentiation coefficient (Gst) was 0.252 8. UPGMA clustering showed that the genetic similarity coefficient was 0.59 ~ 0.99. Conclusion The genetic diversity of Ophiopogon japonicus is high and the genetic variation is mainly in the population.