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目的：观察兔心肌缺血再灌注前后ＰＭＮ（中性粒细胞）的活性变化过程。了解银杏叶提取物保护心肌是否与影响中性粒细胞活化作用有关。方法：取３０ 只日本大耳白兔随机均分为３ 组，假手术对照组、缺血再灌注组和实验用药组。用药方法：用自制的银杏叶提取物注射剂（总黄酮含量５ｍ ｇ／ｍ ｌ）５ｍ ｌ静滴给药。分别于冠脉结扎前和结扎缺血１ｈ 后采血用布式显微镜观察白细胞活化状态的变化。检测白细胞表面粘附分子ＣＤ１１／ＣＤ１８ 表达率、血浆ＭＤＡ（丙二醛）、ＣＴｎｌ（肌钙蛋白１）。结果：在布式显微镜下白细胞活化状态的变化是：冠脉结扎前活化的中性粒细胞（ＰＭＮ）不超过５％ ，结扎１ｈ 后活化的白细胞达４０％ ～６０％ ，而给药组结扎１ｈ 后活化的白细胞不超过２０％ 。表面粘附分子ＣＤ１１／ＣＤ１８表达率的变化趋势与布式显微镜下白细胞活化状态的变化相一致。心肌缺血再灌注后血浆ＭＤＡ、ＣＴｎｌ含量明显升高，ＧＢＥ组血浆ＭＤＡ、ＣＴｎＩ含量明显低于缺血再灌注组。结论：心肌缺血再灌注时，存在着有ＰＭＮ 介导的心肌缺血再灌注损伤，ＰＭＮ 在缺血再灌注后心肌损伤中起重要的作用。布氏显微镜动态观察ＰＭＮ 活化状况，是了解ＰＮＭ 功能的一个简单、直接有效的方法，
Objective: To observe the changes of activity of PMN (neutrophil) before and after myocardial ischemia-reperfusion in rabbits. Understanding whether Ginkgo biloba extract protects myocardium is related to the effects of neutrophil activation. METHODS: Thirty Japanese white rabbits were randomly divided into three groups: sham-operated control group, ischemia-reperfusion group, and experimental drug group. Method of administration: Intravenous administration of a homemade Ginkgo biloba extract injection (total flavonoid content 5m g/ml) 5ml. The changes of leukocyte activation status were observed by cloth microscopy after coronary artery ligation and ligation for 1 h. The expression of CD11/CD18 on the surface of leukocytes, plasma MDA (malondialdehyde), and CTnl (troponin 1) were measured. RESULTS: Changes in the activation state of leukocytes under a cloth microscopy were: activated neutrophils (PMN) before coronary artery ligation did not exceed 5%, activated leukocytes after ligation for 1 h reached 40% to 60%, and the administration group was ligated. After 1h no more than 20% of activated white blood cells. The change of the expression rate of surface adhesion molecule CD11/CD18 was consistent with the change in the activation state of leukocytes under a cloth microscope. Plasma MDA and CTnl levels were significantly increased after myocardial ischemia and reperfusion, and plasma MDA and CTnI levels were significantly lower in the GBE group than in the ischemia-reperfusion group. Conclusion: There is PMN-mediated myocardial ischemia-reperfusion injury during myocardial ischemia and reperfusion. PMN plays an important role in myocardial injury after ischemia-reperfusion. The dynamic observation of PMN activation by the Brookfield microscope is a simple, straightforward and effective way to understand the PNM function.