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用二个籼型杂交组合的F_2群体,构建了二张分别具有89和93个标记位点的RFLP图谱。在此基础上,应用方差分析法和区间作图法对控制籼稻粒形性状的QTLs进行定位。在特三矮2号/CB1128群体中,定位了14个QTLs,其中5个控制粒长,2个主效基因(分别位于第5、第7染色体)和2个微效基因控制粒宽,1个主效基因和4个微效基因控制粒厚。在外引2号/CB1128群体中,共定位了13个QTLs,其中5个影响粒长,2个主效基因(分别位于第2、第5染色体)和3个微效基因控制粒宽,3个微效基因影响粒厚。此外,估算了每个QTL的贡献率、加性效应和显性效应值,分析某些染色体区间的多效现象,比较分析二群体QTLs定位结果的异同。
Two RFLP maps with 89 and 93 marker sites were constructed with two F2 populations of indica hybrids. On the basis of this, the QTLs controlling the grain traits of indica rice were located using the method of analysis of variance and interval mapping. In Tejiangzai 2 / CB1128 population, 14 QTLs were mapped, of which 5 were controlling grain length, 2 major genes (located on chromosomes 5 and 7) and 2 minor genes controlling grain width and 1 A major gene and four micro-gene control grain thickness. A total of 13 QTLs were mapped in the population of Citing / CB1128, of which 5 affected grain length, 2 major genes (located on chromosomes 2 and 5), 3 control genes Mimicry effect grain thickness. In addition, the contribution rate, additive effect and dominance effect value of each QTL were estimated, the effect of multiple chromosome intervals was analyzed, and the similarities and differences of QTLs mapping results of two populations were compared.