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肌动蛋白解聚合因子(actin-depolymerizing factor, ADF)是一种在真核生物中广泛存在的低分子量的肌动蛋白结合蛋白,它在调控细胞内肌动蛋白纤丝的解聚合和再聚合中起着关键作用。我们在棉纤维cDNA文库中分离克隆了4个ADF基因(cDNAs),分别命名为GhADF2,GhADF3,GhADF4,GhADF5。GhADF2 cDNA 长度为705 bp,编码139个氨基酸;GhADF3 cDNA长度为819 bp,编码139个氨基酸;GhADF4 cDNA长度为804 bp,编码143个氨基酸;GhADF5 cDNA长度为644 bp,编码141个氨基酸。分析表明,GhADF2与GhADF3的氨基酸序列同源性为99%。而且,GhADF2/3与矮牵牛PeADF2之间的氨基酸序列同源性也高达89%。GhADF4与拟南芥AtADF6的亲缘关系较近,二者的氨基酸序列同源性为78%。GhADF5与拟南芥AtADF5的亲缘关系较近,氨基酸序列的同源性为83%。上述结果表明植物ADF基因在进化中具有高度保守性。RT-PCR分析表明,GhADF2在纤维中优势表达,而GhADF5基因则在子叶中表达量最高。另一方面,GhADF3和GhADF4似乎不具有组织特异性或偏爱性表达。同一组织中不同GhADF基因表达量有较大的差异,表明它们可能涉及棉花不同组织生长发育过程的调节。而且,在进化过程中,各ADF同分异构体之间可能发展形成某种功能上的差异性。
Actin-depolymerizing factor (ADF) is a low-molecular-weight actin-binding protein that is widespread in eukaryotes and plays an important role in depolymerization and repolymerization of intracellular actin filaments Play a key role. We isolated and cloned four ADF genes (cDNAs) from cotton fiber cDNA library and named GhADF2, GhADF3, GhADF4 and GhADF5 respectively. The length of GhADF2 cDNA was 705 bp, encoding 139 amino acids. The length of GhADF3 cDNA was 819 bp, encoding 139 amino acids. The length of GhADF4 cDNA was 804 bp, encoding 143 amino acids. The length of GhADF5 cDNA was 141 amino acids. The analysis showed that the amino acid sequence homology of GhADF2 and GhADF3 was 99%. Moreover, the amino acid sequence homology between GhADF2 / 3 and petunia PeADF2 is as high as 89%. GhADF4 and Arabidopsis AtADF6 closer genetic relationship, the two amino acid sequence homology was 78%. GhADF5 and Arabidopsis AtADF5 closer genetic relationship, the amino acid sequence homology was 83%. The above results indicate that plant ADF gene is highly conserved in evolution. RT-PCR analysis showed that GhADF2 was predominantly expressed in the fiber, whereas GhADF5 was the highest expressed in cotyledons. On the other hand, GhADF3 and GhADF4 do not seem to have tissue-specific or favored expression. The differences of GhADF gene expression in the same tissues show that they may involve in the regulation of the growth and development of different tissues of cotton. Moreover, there may be some functional differences between the various ADF isomers during evolution.