用铀、铅对超薄切片染色可增强电子反差,但有时会出现沉淀,当出现沉淀时,要重新切片和染色,甚至更换染液,不但耗时、耗力,且某些定位的结构在新片中难以重现。为解决上述问题,我们根据Kuo法,用不同浓度的冰醋酸水溶液,对消除超薄切片染色沉淀进行了实验,获得满意的结果,现介绍如下:1 材料和方法1.1 处理液配制 1.7 mol/L,3.3 mol/L冰醋酸水 Uranium, lead on the ultra-thin slices can enhance the electronic contrast, but sometimes precipitate, when the precipitation, to re-slice and stain, or even replace the dye, not only time-consuming and labor-consuming, and some of the structure of the positioning The new film is difficult to reproduce. In order to solve the above problems, according to the Kuo method, with different concentrations of glacial acetic acid aqueous solution, to eliminate ultra-thin section staining precipitation experiments were satisfactory results are as follows: 1 Materials and methods 1.1 Preparation of liquid preparation 1.7 mol / L , 3.3 mol / L glacial acetic acid water