本文采用分裂素自养型中国红豆杉细胞株 ,研究了在细胞悬浮培养过程中茉莉酸甲酯 ( MJ)对紫杉醇生物合成的诱导作用。结果表明 ,以乙醇为 MJ助溶剂时 ,MJ的诱导作用以剂量为 2 0 0 μmol/L于继代培养开始时加入为最佳 ,此时紫杉醇产量较对照组提高 71.2 %。以吐温为 MJ助溶剂时 ,MJ的诱导作用以剂量为 10μmol/L于继代培养 d2 0加入为最佳 ,此时紫杉醇产量较对照组提高 2 80 .7%。此外 ,本文对 MJ的诱导作用机理进行了探讨。 In this paper, mitogen-induced autotrophic yew cell lines were used to study the induction of paclitaxel biosynthesis by methyl jasmonate (MJ) during cell suspension culture. The results showed that when ethanol was used as MJ cosolvent, the induction of MJ was best when the dosage of 200 μmol / L was added at the beginning of subculture, and the yield of paclitaxel was increased by 71.2% compared with the control. When Tween was used as MJ cosolvent, the induction of MJ was best when the dose of 10μmol / L was added into the subcultured d2 0, and the yield of paclitaxel increased by 280.7% compared with the control group. In addition, the mechanism of induction of MJ was discussed in this paper.