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本研究利用PCR技术克隆了大豆Gm Gol S2基因的启动子序列。系统进化树分析表明,Gm Gol S2与沙冬青的Gols亲缘关系最近。利用Plant CARE分析启动子元件发现,在Gm Gol S2基因启动子序列中含有多个与逆境胁迫、激素等反应相关的元件。在The Bio-Analytic Resource for Plant Biology数据库中分析了Gm Gol S2的同源基因At Gol S2在不同逆境胁迫处理时的基因表达情况,结果表明该基因具有组织表达特异性,并参与盐胁迫、渗透胁迫及干旱胁迫等反应。RT-PCR结果表明Gm Gol S2基因受干旱诱导上调表达。
In this study, the promoter of soybean Gm Gol S2 gene was cloned by PCR. Phylogenetic tree analysis showed that Gm Gol S2 had the closest relationship with Gols of Ammopiptanthus mongolicus. Analysis of promoter elements using Plant CARE revealed that there are several elements involved in response to stress, hormones and other factors in the Gm Gol S2 gene promoter sequence. The gene expression of At Gol S2, a homologous gene of Gm Gol S2, under different stress conditions was analyzed in The Bio-Analytic Resource for Plant Biology database. The results show that the gene has tissue-specific expression and is involved in salt stress, Stress and drought stress response. RT-PCR results showed that Gm Gol S2 gene was up-regulated by drought.