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以干热３００ ℃作用于钢片表面枯草杆菌黑色变种芽胞，以２ ％ 戊二醛溶液作用于布片上的枯草杆菌黑色变种芽胞，以１２１ ±０ ．５ ℃压力蒸汽作用于纸片上嗜热脂肪杆菌芽胞。采用定性与定量试验分别检测杀灭效果。结果，定性试验达到无菌所需作用时间，均较定量试验杀灭率达１００ ％ 者为长。
The effect of dry heat 300 ℃ on the black surface of Bacillus subtilis spores, with 2% glutaraldehyde solution on the cloth of Bacillus subtilis black mutant to 121 ± 0. 5 ℃ pressure steam on the role of fatty acid Bacillus spores cells. Qualitative and quantitative tests were used to detect the killing effect. As a result, the time required for qualitative tests to achieve sterility was longer than 100% for the quantitative test.