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目的探究位于Let-7与肺癌基因RAS基因3’UTR(KRAS3’UTR)互补区中的rs712是否影响Let-7与肺癌基因RAS结合,从而影响Let-7对肺癌基因的调控及非小细胞肺癌(NSCLC)患病风险的关系。方法取83例原发性NSCLC癌患者肺的组织和血液样本(实验组),80例正常组织和血液样本(对照组),采用PCR方法扩增KRAS3’UTR中包含rs712区域的基因片段,扩增产物以限制性酶切(RFLP)及凝胶电泳进行基因分型。结果 rs712的基因型与等位基因频率在实验组与对照组的差异无统计学意义(P>0.05);rs712的基因型频率和等位基因频率在NSCLC各个分期型、不同组织病理间的差异无统计学意义(P>0.05)。结论在NSCLC患者中,rs712与NSCLC的患病风险没有明显关系。结合区域中单核苷酸多态性位点(SNP)基因分型是否成为患病风险的检测方法之一,是否能帮助肿瘤的早期诊断、肿瘤分型、预测肿瘤复发、转移和判断预后提供重要的信息还需要进一步的研究。
Objective To investigate whether rs712 located in the complementary region of Let-7 and KRAS3’UTR of RAS gene affects the combination of Let-7 and lung cancer gene RAS, thus affecting the regulation of Let-7 on lung cancer genes and non-small cell lung cancer (NSCLC) the risk of the relationship. Methods Tissue and blood samples of 83 NSCLC patients with lung cancer (experimental group) and 80 normal controls and blood samples (control group) were collected. The gene fragment of rs712 in KRAS 3 ’UTR was amplified by PCR. The amplified product was subjected to restriction analysis (RFLP) and gel electrophoresis for genotyping. Results There was no significant difference in genotype and allele frequencies of rs712 between the experimental group and the control group (P> 0.05). The frequencies of rs712 genotypes and alleles in all staging and pathological changes of NSCLC No statistical significance (P> 0.05). Conclusion There is no significant relationship between rs712 and the risk of NSCLC in NSCLC patients. Whether SNP genotyping in the binding region is one of the test methods for determining the risk of a disease can help in the early diagnosis of tumors, tumor typing, prediction of tumor recurrence, metastasis and judgment of prognosis Important information still needs further study.