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目的 探讨丙型病毒性肝炎患者血清HCV-RNA载量与抗-HCV浓度检测的临床意义.方法 采用荧光定量PCR对本院2016年1月-2017年7月就诊的113例丙型病毒性肝炎患者和30例健康对照进行HCV-RNA载量检测并对基因组测序分型,同时使用ELISA检测抗-HCV浓度,全自动生化分析仪检测血清AST、ALT、TBIL浓度.结果 陕西榆林地区丙肝患者主要基因型为HCV1型82例(72.57％);2型10例(8.85％);3型5例(4.42％);6型7例(6.19％).随着HCV-RNA的增加,抗-HCV阳性率有所增高,两者一致性增高.丙肝患者抗-HCV的S/CO值均高于健康对照组,差异有统计学意义.HCV-RNA四个载量水平不同组间除中、低载量组AST无统计学意义外,ALT、AST、TBIL水平比较差异均有统计学意义.结论 HCV-RNA联合抗-HCV检测与肝功能相关指标(AST、ALT、TBIL)检测三者相互结合,对于丙型病毒性肝炎患者的早期诊断和疗效监测具有重要的意义.“,”Objective:To investigate the clinical value of detecting the HCV-RNA and Anti-HCV in patients with Hepatitis C.Methods:113 cases of hepatitis C patients in hospital were selected as the object of study from January 2016 to July 2017.30 cases of healthy person were selected.The HCV-RNA load and Anti-HCV of all patients and healthy person were detected by fluorescence quantitative PCR and ELISA respectively.Sanger sequencing was used for analyzing HCV genotype.THE ALT、AST and TBIL indicators were measured by automatic biochemical analyzer.Results:The main genotypes of hepatitis c in the Yulin area ofshaanxi were HCV1 (72.57％),HCV2 (8.85％),HCV3 (4.42％),HCV6 (6.19％).With the increase of HCV-RNA,the anti-HCV positive rate were increased.The S/CO value of anti-HCV was higher than that in the healthy control group,and the difference was significant.The differences of ALT,AST,and TBIL were statistical significance in 4 groups of HCV-RNA except for the AST in low-load and mid-load.Conclusion:The combination of HCV-RNA、anti-HCV detection and the detection of liver function detection has an important guiding value for early diagnosis,treatment and prognosis of patients with hepatitis C.