The Effect of Different Types of Filters on the Fluorometric Determination of Chlorophyll a in Seawa

来源 :Acta Oceanologica Sinica | 被引量 : 0次 | 上传用户:bbyyqq555
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A comparative study using fluorometry on 6 types of filters for the determination ofChl a in seawater was carried out. The results show that whatman GF/C glass fiber filterwith a layer of MgCO_3 is the best among them. The values of Chl a obtained by thisway were not only higher, but transparency of extracts was also better than that by otherfilters. The sensitivity of fluorometry was much higher than that of spectrophotometry, sothat only small amount of seawater was enough. Furthermore, the grinding and centrifuga-tion can be eliminated, so the procedure is much simplified and is suitable to be used onboard ship. A comparative study using fluorometry on 6 types of filters for the determination of Chl a in seawater was carried out. The results show that whatman GF / C glass fiber filter with a layer of MgCO_3 is the best among them. The values ​​of Chl a obtained by thisway were not only higher, but transparency of extracts was also better than that by otherfilters. The sensitivity of fluorometry was much higher than that of spectrophotometry, sothat only small amount of seawater was enough. Furthermore, the grinding and centrifuga- tion can be eliminated, so the procedure is much simplified and is suitable to be used onboard ship.
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