论文部分内容阅读
目前国内外虽已有对HBVDNA和INVRNA用PCR方法一次性同时检测技术．但由于方法复杂．易污染．费用高，在基层医院难以开展．笔者根据HBVDNA在血清中含量较多．易于提取的特点，将HBV的引物用量由两次扩增减为一次扩增．并将蛋白酶K改为异硫氰酸胍一饱和酚抽提，使反应时间有所缩短，又使成本费减少近30％。经对92例患者血清的检测．结果发现与同份血清单一PCR方法检测HBV和HCV感染阳性的总符合率为98．16％．与国内、外文献报道的乙、丙型肝炎患者血清中HBVDNA和HCVRNA阳性率相符合、为了进一步验证该方法的可靠性．又对30例随机抽样患者的同份血清进行了两种方法调换检测顺序的检测．其两种方法的阳性符合率为100％。说明该方法在检测时间，节省人力、物力．减少污染，提高基层医院应用PCR技术的方便程度上都有较大优越性，前景是乐观的。
Although at home and abroad, there has been one-time simultaneous detection of HBVDNA and INVRNA by PCR method. However, due to the complicated method. Easy to pollute. High cost, difficult to carry out in primary hospitals. The author based on HBVDNA content in the serum more. Easy to extract features, the amount of primers for HBV from two amplification to an amplification. And the protease K was changed to guanidine isothiocyanate-saturated phenol extraction, the reaction time is shortened, but also reduce the cost of nearly 30%. After 92 cases of patients with serum testing. The results showed that with the same sera of single PCR detection of HBV and HCV infection in the total positive rate was 98.16%. It is consistent with the positive rates of HBVDNA and HCV RNA in the serum of patients with hepatitis B and C reported in the domestic and foreign literature, in order to further verify the reliability of the method. Again 30 cases of random samples of patients with the same sera for two methods to change the test sequence detection. The positive coincidence rate of the two methods was 100%. Description of the method in the detection of time and save manpower and material resources. Reduce pollution and improve the convenience of primary hospital PCR technology has greater advantages, the outlook is optimistic.