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目的探讨失血性休克(简称休克)大鼠肠上皮细胞核转录因子mtTFA基因以及核编码线粒体蛋白基因COX Ⅳ和线粒体编码COXⅠ基因表达的改变。以探讨在缺血缺氧性损害中核转录因子mtTFA对线粒体基因组表达的影响。方法采用RT-PCR方法观察休克大鼠肠上皮细胞mtTFA、COXⅠ和COXⅣ基因mRNA量的改变。结果休克早期mtTFA mRNA表达变化不明显,至休克4 h有所增强,到休克5 h下降到休克前水平;休克1 h大鼠肠上皮细胞COXI mRNA表达开始增加,3 h达高峰,后又降低,到休克5 h显著低于休克前水平。休克大鼠肠上皮细胞核基因编码COXⅣmRNA休克3 h略有增高,休克4 h后开始下降,至休克晚期低于休克前水平。结论 mtTFA mRNA表达变化在休克时发生较晚,且升高幅度较小,说明休克时该基因对细胞缺血缺氧性损害的敏感性较低。休克时COX Ⅰ mRNA比COX Ⅳ mRNA上调速度快,维持时间长,说明休克早期线粒体基因表达的改变在细胞缺血缺氧性损伤的保护中起着更重要的作用。
Objective To investigate the changes of mitochondrial COX Ⅰ gene expression and mtTFA gene in the intestinal epithelial cells of rats with hemorrhagic shock (abbreviated as shock). To investigate the effect of nuclear transcription factor mtTFA on mitochondrial genome expression in hypoxic-ischemic damage. Methods The changes of mtTFA, COX Ⅰ and COX Ⅳ mRNA in the intestinal epithelial cells of shock rats were observed by RT-PCR. Results The mRNA expression of mtTFA in early stage of shock did not change obviously, increased to the level of 4 h after shock, and decreased to the level before shock 5 h after shock; the expression of COXI mRNA in intestinal epithelial cells began to increase 1 h after shock and peaked at 3 h, , 5 h after shock was significantly lower than the level before shock. Shock rat intestinal epithelial cells encoding COX ¢ ômRNA shock increased slightly after 3 h, shock began to decline after 4 h, to the shock before the end of shock was lower than the level before shock. Conclusion The change of mtTFA mRNA expression occurs later in shock, and the increase is smaller, indicating that this gene is less susceptible to hypoxic-ischemic cell damage during shock. COX Ⅰ mRNA up-regulated faster than COX Ⅳ mRNA in shock and maintained for a long time, indicating that mitochondrial gene expression in early stage of shock plays a more important role in the protection of hypoxic-ischemic injury.