从食管癌高发区林县农户粮食中分离出19株圆弧青霉,随机选4株(45A_2,7B_3,155D_1,26B_1),在以甘露醇为碳源的Raulin-Thom培养基中培养。氯仿提取其代谢物,应用CHO(Chinese hamster ovary)细胞SCE(Sister Chromatid Exchange)法检测了它们的诱变作用,结果发现:45A_2加S_9后诱发SCE频率显著升高;7B_3,155D_1在不加S_9情况下诱发SCE频率显著升高;26B_1在加与不加S_9情况下均为阳性,但以不加S_9时作用较强,所测试的4株圆弧青霉代谢物均有不同程度的损伤细胞DNA作用,与食管癌的发生可能有一定关系。 19 strains of Penicillium circular arctii were isolated from the farmer households in Linxian, a high incidence area of ​​esophageal cancer. Four strains (45A_2, 7B_3, 155D_1 and 26B_1) were randomly selected and cultured in Raulin-Thom medium with mannitol as carbon source. Their metabolites were extracted by chloroform and their mutagenicity was tested by SCE (Schatero Chromatid Exchange) method. The results showed that the frequency of SCE was significantly increased after 45 A_2 addition of S_9, but not in the absence of S_9 SCE induced a significant increase in the frequency of SCE; 26B_1 with and without S_9 cases were positive, but with no strong role S9, the four tested Penicillium citricum metabolites have varying degrees of damaged cells DNA role, and esophageal cancer may have a certain relationship.