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对基因型、小孢子发育时期、高温起始培养、培养基等甘蓝型油菜游离小孢子培养诱导胚状体的主要影响因素，研究表明：基因型和小孢子发育时期起决定性的作用，不同品种间甚至同一品种内的个体间，诱导频率差异显著；适宜培养的小孢子发育时期为单核晚期；培养基中加入活性炭可提高胚状体诱导频率；起始培养高温热击促进胚状体诱导。成熟胚状体在无激素Ｂ５培养基中，直接再生出植株或经次生胚继代培养再生植株。
The main influencing factors on the induction of embryoid body from microspore culture of Brassica napus such as genotype, microspore development, high temperature initial culture and medium were studied. The results showed that genotype and microspore development play a decisive role, Even among individuals within the same species, the induction frequency was significantly different; suitable microspore developmental stage was mononuclear stage; activated carbon was added to the medium to increase the induction frequency of embryoid body; . Mature embryoid bodies in hormone-free B5 medium, regenerated directly from the plant or secondary embryo regeneration of regenerated plants.