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用自制的簧片打击装置弹击大鼠枕骨结节，造成严重颅脑损伤或原发性脑干伤，取其脑干（中脑、桥脑及延髓）常规包埋、制片后作LSAB－FN染色。结果表明，在严重颅脑损伤及原发性脑干伤，可见FN沉积于血管周围间质、部分神经纤维内及一些神经细胞内，且随着损伤时间的延长，FN沉积量增加、范围增大。LSAB－FN法可用于生前原发性脑干伤与死后伤的鉴别和推断在3小时内损伤经过时间。
Using self-made reed strike device to hit the occipital nodules in rats, resulting in severe brain injury or primary brain stem injury, the brain stem (midbrain, pons and medulla oblongata) were routinely embedded after making LSAB -FN staining. The results showed that in severe traumatic brain injury and primary brain stem injury, FN deposition in the perivascular stroma, some nerve fibers and some nerve cells, and with the extension of injury time, FN deposition increased by a Big. LSAB-FN method can be used to identify and infer the injury of primary brain stem injury and post-mortem injury within a lifetime of damage within 3 hours.