目的探讨γ干扰素（ＩＦＮγ）基因修饰的肝细胞经脾移植抗血吸虫病肝纤维化作用。方法采用ＥＬＩＳＡ和免疫组化方法，动态分析日本血吸虫感染小鼠ＩＦＮγ和Ⅰ、Ⅲ型胶原水平及其相互关系，并将小鼠ＩＦＮγ重组腺病毒转染的肝细胞经脾移植到感染１６周小鼠，分析ＩＦＮγ基因治疗血吸虫病肝纤维化的作用。结果感染４周时，小鼠ＩＦＮγ水平明显升高，在感染１２周时达高峰；但在１６周以后ＩＦＮγ水平明显下降，而Ⅰ、Ⅲ型胶原的合成持续增高；ＩＦＮγ基因修饰的肝细胞经脾移植后能稳定有效地表达，显著降低Ⅰ、Ⅲ型胶原的合成、沉积，减轻肝纤维化程度。结论ＩＦＮγ可能与Ⅰ、Ⅲ型胶原的合成、沉积有关；ＩＦＮγ基因治疗能有效地发挥抗血吸虫病肝纤维化作用。 Objective To investigate the anti-liver fibrosis effect of interferon-γ (IFNγ) gene modified hepatocyte transplantation on splenic schistosomiasis. Methods ELISA and immunohistochemical methods were used to dynamically analyze the levels of IFNγ and collagen type Ⅰ and Ⅲ in mice infected with Schistosoma japonicum and the correlation between the expression of IFNγ and the collagen type Ⅰ collagen. Infection 16 weeks mice, analysis of IFN γ gene treatment of schistosomiasis liver fibrosis. Results At 4 weeks after infection, the level of IFNγ in mice increased significantly and peaked at 12 weeks of infection. However, the levels of IFNγ decreased significantly after 16 weeks, while the synthesis of type Ⅰ and Ⅲ collagen continued to increase. IFNγ gene modification Of hepatocytes stably and effectively expressed after spleen transplantation, significantly reducing the synthesis and deposition of type I and type III collagen, and reducing the degree of liver fibrosis. Conclusions IFN  γ may be related to the synthesis and deposition of type Ⅰ and type Ⅲ collagen. IFN  γ gene therapy can effectively inhibit liver fibrosis induced by schistosomiasis.