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目的 探讨新型基因工程肿瘤坏死因子(nrhTNF)和足叶乙甙(VP16)的协同抗瘤作用,及nrhTNF的毒副作用。方法 以复制成功的Lewis肺癌(3LL)小鼠为模型。结果 显示nrhTNF或VP16瘤内注射均能使肿瘤生长及肺转移受到一定的抑制(抑瘤率分别为33.71％。30.46％,肺转移瘤数与对照组比较P<0.05),肿瘤出现一定程度坏死,而联合应用则显著抑制肿瘤生长及肺转移(抑瘤率65.77％,肺转移瘤数与对照组比较P<0.01),肿瘤出现广泛的出血坏死,同时观察到nrhTNF对实验小鼠无明显毒副作用。结论 本研究表明:nrhTNF和VP16联合应用,具有协同抗肿瘤作用。对于肺癌治疗具有进一步临床应用探讨价值。
Objective To investigate the synergistic anti-tumor effect of novel genetically engineered tumor necrosis factor (nrhTNF) and etoposide (VP16) and the toxic effects of nrhTNF. Methods The successful replication of Lewis lung carcinoma (3LL) mice as a model. The results showed that intratumoral injection of nrhTNF or VP16 could both inhibit tumor growth and lung metastasis (tumor inhibition rates were 33.71% and 30.46%, respectively, and the number of lung metastases was significantly lower than that of the control group (P <0.05). The tumor developed a certain degree of necrosis , While the combined application significantly inhibited tumor growth and lung metastasis (tumor inhibition rate was 65.77%, the number of lung metastases compared with the control group P <0.01), extensive hemorrhage and necrosis occurred in the tumor, and nrhTNF was observed in experimental mice without obvious toxicity side effect. Conclusion This study shows that: nrhTNF and VP16 combined with a synergistic anti-tumor effect. For lung cancer treatment has further clinical value.