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观察了不同浓度牛血浆纤维粘连蛋白（ＦＮ）对培养的人牙髓成纤维细胞Ⅲ型胶原、ＦＮ表达的影响；用图象分析仪对染色强弱进行相对定量分析，ＦＮ（１０、２０μｇ／ｍｌ）组的人牙髓细胞Ⅲ型胶原表达最强，ＦＮ（４０，８０μｇ／ｍｌ）组的人牙髓细胞ＦＮ表达最强，说明ＦＮ的不同浓度影响了培养的人牙髓成纤维细胞的功能，ＥＬＩＳＡ测定结果可做为图象分析的参考与补充。
The effects of different concentrations of bovine fibronectin (FN) on the expression of type Ⅲ collagen and fibronectin in cultured human dental pulp fibroblasts were observed. Relative quantification of the staining intensity was carried out by image analyzer. FN (10, 20 μg / ml) had the strongest expression of type III collagen in human dental pulp cells. FN (40,80μg / ml) group had the strongest expression of FN in human dental pulp cells, which indicated that the different concentrations of FN influenced the proliferation of cultured human dental pulp fibroblasts Function, ELISA test results can be used as a reference image analysis and complement.