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目的研究谷氨酸（Ｇｌｕ）对皮质神经元的损伤及对Ｃａ２＋／ＣａＭＰＫⅡ活性的影响。方法５００μｍｏｌ／ＬＧｌｕ作用１０ｍｉｎ，测Ｃａ２＋／ＣａＭＰＫⅡ及乳酸脱氢酶（ＬＤＨ）活性。结果Ｃａ２＋／ＣａＭＰＫⅡ活性和正常对照相比下降４６．３％，１００μｍｏｌ／Ｌ氯胺酮几乎完全保护该酶活性；ＬＤＨ活性在１ｈ时无明显变化，２４ｈ时明显升高。结论（１）Ｇｌｕ对皮质神经元损伤致Ｃａ２＋／ＣａＭＰＫⅡ活性下降早于ＬＤＨ变化；（２）Ｃａ２＋／ＣａＭＰＫⅡ活性下降主要由ＮＭＤＡ受体介导，与非ＮＭＤＡ受体无关。
Aim To study the effects of glutamate (Glu) on cortical neurons and their effects on Ca2 + / CaMPKⅡ activity. Methods The activity of Ca2 + / CaMPKⅡ and lactate dehydrogenase (LDH) were measured after 500μmol / L LGU for 10min. Results The Ca2 + / CaMPKⅡ activity decreased by 46.3% compared with that of the normal control. Ketamine at 100μmol / L almost completely protected the activity of the enzyme. The activity of LDH did not change significantly at 1h, but significantly increased at 24h. Conclusions (1) Glu decreased the activity of Ca2 + / CaMPKⅡin the cortical neurons and decreased earlier than LDH. (2) The decrease of Ca2 + / CaMPKⅡactivity was mainly mediated by NMDA receptor, but not with non-NMDA receptor.