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目的通过观察香烟提取物对牛冠状动脉内皮细胞(BCAEC)的损伤作用,为研究吸烟与心血管疾病之间的关系提供依据。方法以吸烟者体内通常的尼古丁浓度为基准,分别用尼古丁、香烟主流烟雾提取物(MSW)和侧流烟雾提取物(SSW)对 BCAEC 进行染毒(尼古丁染毒终浓度分别为1×10~(-5)、0.8×10~(-5)、0.9×10~(-5)mol/L),用显微数码成像系统记录细胞形态变化,检测凋亡细胞与坏死细胞的发生比例,测定 caspase 酶活力。结果经尼古丁、MSW 处理的 BCAEC 形态上发生凋亡样变化,处理24 h 后凋亡细胞发生率为5.89%和11.94%。而 SSW 处理后的 BCAEC 呈坏死样变化.处理24 h 后62.84%的细胞发生坏死。尼古丁和 MSW 可诱导 caspase-3活力增高。结论香烟提取物可诱导牛冠状动脉内皮细胞损伤,MSW 可引起细胞凋亡,SSW 导致细胞坏死,caspase-3的激活可能是香烟提取物诱导牛冠状动脉内皮细胞凋亡的机制。
Objective To investigate the effect of cigarette extract on the damage of bovine coronary artery endothelial cells (BCAEC), and to provide a basis for studying the relationship between smoking and cardiovascular diseases. Methods Nicotine, mainstream tobacco smoke extract (MSW) and sidestream smoke extract (SSW) were used to treat BCAEC on the basis of the usual concentration of nicotine in smokers. The final concentrations of nicotine were 1 × 10 ~ (-5), 0.8 × 10 -5 and 0.9 × 10 -5 mol / L, respectively. The morphological changes of cells were recorded by microscopic digital imaging system, and the ratio of apoptotic cells to necrotic cells was measured. caspase activity Results The apoptotic changes of BCAEC treated with nicotine and MSW were observed. The apoptosis rate of apoptotic cells was 5.89% and 11.94% after 24 h. However, the necrotic changes of BCAEC after SSW treatment showed that 62.84% of the cells died after 24 h treatment. Nicotine and MSW induced an increase in caspase-3 activity. Conclusion Cigarette smoke extract can induce the injury of bovine coronary artery endothelial cells. MSW can induce cell apoptosis. SSW leads to cell necrosis. The activation of caspase-3 may be the mechanism of tobacco extract inducing bovine coronary artery endothelial cell apoptosis.