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目的：建立五味子含药血清中主要木脂素类成分含量测定的方法。方法：采用HPLC法测定，色谱柱为Lichrosphere C18（4.6 mm×250 mm，5μm），柱前加Phenomenex R Description C18（4.0 mm×3.0 mm）保护柱，水-乙腈为流动相，梯度洗脱，流速为1.0 mL/min，柱温为30℃，检测波长为210 nm。结果：4种木脂素类成分五味子醇甲、醇乙、甲素和乙素分别在0.0512-0.7680μg、0.0540-0.8100μg、0.0123-0.1845μg和0.0398-0.5970μg范围内线性关系良好，相关系数r分别为0.9995、0.9996、0.9998和0.9996，含药血清中4种木脂素类成分的平均含量分别为19.3848、13.2624、1.3001和14.5842μg/mL。结论：该法准确、简便、专属性强，可用于五味子含药血清中主要木脂素类成分的测定。“,”Objective: To establish a method for the assaying of main lignans in medicated serum of Schisandra chinensis. Methods: An HPLC method was set up, and Lichrosphere C18 column(4.6 mm×250 mm, 5 μm) was used and PhenomenexR De-scription C18 (4.0 mm×3.0 mm) was used as protective column. The gradient mobile phase was acetonitrile-water. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and the detection wavelength was 210 nm. Results: The linear ranges of schisandrin, schisandrol B, deoxyschisandrin, and schisandrin B were respectively 0.0512 -0.7680 μg, 0.0540 -0.8100 μg, 0.0123-0.1845 μg, and 0.0398-0.5970 μg, and the coefficient correlation (r) were 0.9995, 0.9996, 0.9998, and 0.9996, respec-tively. The average concentration of these 4 lignans in medicated serum were 19.3848, 13.2624, 1.3001, and 14.5842 μg/mL, respectively. Conclusion: This method is accurate, easy, and specific for the assaying of lignans in medicated serum of Schisandra chinensis.