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目的制备抗呼吸道合胞病毒（ＲＳＶ）的单克隆抗体，并对其特性进行系统地鉴定。方法以活的ＲＳＶＬｏｎｇ株滴鼻（ＴＣＩＤ５０为１×１０－６，取１００ＴＣＩＤ５００１２５ｍｌ进行）加ＲＳＶ感染Ｈｅｐ２细胞（ＣＰＥ达～）腹腔注射免疫Ｂａｌｂ／ｃ鼠，用细胞融合技术制备能稳定分泌抗ＲＳＶ的单克隆抗体细胞株。结果细胞融合一次成功，融合率为９６％，抗ＲＳＶ抗体阳性孔率为６５％。鉴定１株杂交瘤细胞Ｆ３株，染色体数为９８条，抗体亚类属ＩｇＧ１，对ＲＳＶ抗原特异；在无补体存在的情况下其腹水抗体中和效价为１∶１２８，且具融合抑制活性，腹水融合抑制效价为１∶６４。结论Ｆ３株单克隆抗体为抗ＲＳＶＦ蛋白的ＭｃＡｂ。
Objective To prepare monoclonal antibodies against respiratory syncytial virus (RSV) and systematically identify its characteristics. Methods Balb / c mice were immunized intraperitoneally with live RSVLong strain (TCID50 of 1 × 10-6 and 100TCID of 500125ml) plus RSV infected Hep2 cells (CPE ~ ) Preparation of a monoclonal antibody cell strain capable of stably secreting anti-RSV. Results The cell fusion was successful at one time. The fusion rate was 96% and the positive rate of anti-RSV antibody was 65%. A strain of hybridoma F3 was identified with a chromosome number of 98 and a subtype of IgG1, which is specific for RSV antigen. Ascites antibody neutralization titer was 1:128 in the absence of complement, and had fusion inhibitory activity , Ascites fusion inhibition titer 1:64. Conclusion The F3 monoclonal antibody is McAb against RSV F protein.