目的亚克隆免疫筛选日本血吸虫ｃＤＮＡ文库得到的基因。方法在体外将血吸虫ｃＤＮＡ文库基因的ＰＣＲ产物和ｐＧＥＭＴ载体连接，转染大肠杆菌ＸＬ１Ｂｌｕｅ，经抗生素及呈色底物Ｘｇａｌ粗筛，再用限制性内切酶及ＰＣＲ方法进一步鉴定。结果成功地将文库中４个大小不同的日本血吸虫基因片段连接到载体中，获得４个重组子。结论为可能编码保护性抗原的血吸虫基因测序和将其亚克隆入真核表达质粒奠定基础。 Objective To subclone the gene of immune screening of Schistosoma japonicum cDNA library. Methods The PCR product of Schistosoma japonicum cDNA library gene was ligated into pGEMT vector in vitro and then transfected into E. coli XL1Blue. The antibiotic and the chromogenic substrate Xgal were screened by restriction endonuclease and PCR Identification. Results Four Schistosoma japonicum gene fragments in the library were successfully ligated into the vector to obtain 4 recombinants. Conclusion This study laid the foundation for gene sequencing of Schistosoma japonicum that could encode protective antigen and subcloning it into eukaryotic expression plasmid.