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采用GC-MS-DS测定隐孔菌菌丝的挥发油成分,结果表明,在检出20种成分中包括倍半萜类化合物4种,芳香族化合物10种,脂肪族化合物6种,与野生隐孔菌挥发油成分组成相比共有13种成分相同,占检出成分的65%。挥发油含量(2.185%)高于野生隐孔菌挥发油含量(1.412%)。通过TOT(总离子流)百分比测定,芳香族和脂肪族含量均高于野生隐孔菌,萜类含量二者基本相近。比较二者GC色谱扫描出峰时间,除了芳香族化合物出峰时间有差别外,萜类化合物和脂肪族化合物出峰时间基本相似。综合对糖类、氨基酸、脂类及次生产物的成分比分析,提示可以将发酵培养的隐孔菌菌丝作为野生隐孔菌新药源的替代品进行深入研究
GC-MS-DS was used to determine the volatile oil constituents of the mycelium of C.porobius. The results showed that among the 20 components detected, 4 species of sesquiterpenoids, 10 species of aromatic compounds, and 6 species of aliphatic compounds were detected. The composition of the volatile oil in the pore bacterium is the same as that of 13 components, accounting for 65% of the detected components. The content of volatile oil (2.185%) was higher than that of C. crypticola (1.412%). By the TOT (total ion flux) percentage determination, the aromatic and aliphatic contents were higher than those of the Cryptocaryon solani, and the contents of the terpenoids were basically similar. Comparing the peak times of the two GC chromatograms, the peak times of the terpenoids and the aliphatic compounds were similar except that the peak time of the aromatic compounds was different. Comprehensive analysis of the composition ratios of sugars, amino acids, lipids, and subproducts suggests that the fermented Cryptosporidium mycelia can be used as a substitute for a new source of Cryptocaryon cryptosporium.