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成年Ｗｉｓｔａｒ雄性大鼠２０只，实验组１０只，行松果腺摘除术；对照组１０只。术后３０ｄ将实验组、对照组动物各半数在０９：００～１０：００和１６：００～１７：００分别处死。用免疫组化ＡＢＣ法显示视交叉上核内含ＶＩＰ的神经元；微机图像分析仪上测量其光镜下的切面面积及平均免疫反应强度。结果：（１）对照组不同时间处死的动物ＶＩＰ样神经元切面面积０９：００～１０：００大于１６：００～１７：００，呈节律性变化。（２）摘除松果腺后各时间组间ＶＩＰ样神经元切面面积的差异无显著性，提示实验组动物视交叉上核的ＶＩＰ样神经元功能活动的日周节律已发生改变。（３）对照组和实验组动物的ＶＩＰ样神经元平均免疫反应强度在所测的两个时间中均未见明显差异。
Twenty adult Wistar male rats were randomly divided into experimental group (n = 10) and control group (n = 10). 30d after operation, the experimental animals and control animals were killed halfway between 09: 00-10: 00 and 16: 00-17: 00 respectively. The immunohistochemical ABC method was used to show the VIP-containing neurons in the suprachiasmatic nucleus. The cross-sectional area under the light microscope and the mean immune response intensity were measured on the computerized image analyzer. Results: (1) The VIP-like neurons killed at different time points in the control group had a rhythmic change from 09:00 to 10:00 at 16:00 to 17:00. (2) There was no significant difference in the section area of VIP-like neurons between the groups after pineal excision, suggesting that the rhythm of VIP-like neurons in the suprachiasmatic nucleus of experimental group had changed their rhythms. (3) The average immune response intensity of VIP-like neurons in the control group and experimental group showed no significant difference between the two measured time points.