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人CD34~+造血细胞是具有高度自我更新、多向分化及重建长期造血与免疫学功能的独特体细胞。为系统探索CD34~+造血细胞的形态、细胞化学及超微结构特征,新近我们设计组合并建立了CIMS-100-FACS 440无菌二次分选术,可使所获CD34~+造血细胞的纯度达100%。在此基础上,本研究采用Cambri-dge Quantimet 970全自动图像分析仪对光学显微镜、扫描电镜及透射电镜下的CD34~+造血细胞进行了体视学方面的某些探讨,进一步从三维结构信息中深刻揭示CD34~+造血细胞的形态计量学特征。经扫描→模数转换←阴影校正→图像暂存←统计分析等检测,结果表明:CD34~+造血细胞的直径3.490—6.741μm,周长11.776—26.240μm,面积9.565—35.686μm~2,形状因子1.048—1.840,核浆比0.58—0.72,平均光密度0.17675—0.65100,积分光密度2717.217—9870.643。由此可见CD34~+造血细胞的确为非均一细胞群,这可能与CD34~+造血细胞的功能亚群与分化阶段密切相关。据我们所知,这是国际上首次有关人CD34~+造血细胞体视学特征的报道。
Human CD34 ~ + hematopoietic cells are unique somatic cells with a high degree of self-renewal, multidirectional differentiation and reconstruction of long-term hematopoietic and immunological functions. To systematically explore the morphological, cytochemical and ultrastructural features of CD34 ~ + hematopoietic cells, we recently designed a combination of CIMS-100-FACS 440 sterile secondary sorting, the CD34 ~ + hematopoietic cells Purity up to 100%. On this basis, we used the Cambri-dge Quantimet 970 automatic image analyzer to conduct some stereological studies on the CD34 + hematopoietic cells under light microscope, scanning electron microscope and transmission electron microscope, and further analyzed the three-dimensional structure information In the deep revealing the morphology of CD34 ~ + hematopoietic cells. The results of scanning, analog-digital conversion, shading correction, image temporary storage and statistical analysis showed that the diameter of CD34 ~ + hematopoietic cells ranged from 3.490 to 6.741μm, and the perimeter ranged from 11.776 to 26.240μm with an area of 9.565-35.686μm ~ 2. Factor 1.048-1.840, nuclear ratio 0.58-0.72, average optical density 0.17675-0.65100, integral optical density 2717.217-9870.643. This shows that CD34 ~ + hematopoietic cells is indeed a heterogeneous cell population, which may be related to the functional subpopulation and differentiation stage of CD34 ~ + hematopoietic cells. To the best of our knowledge, this is the first international report of the stereological characteristics of human CD34 + hematopoietic cells.