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目的：探讨咖啡因是否可直接引起离体大鼠脊髓释放降钙素基因相关肽（Ｃａｌｃｉｔｏｎｉｎｇｅｎｅ－ｒｅｌａｔ－ｅｄｐｅｐｔｉｄｅ，ＣＧＲＰ）及其作用机制。方法：采用离体大鼠脊髓切片孵育，以放射免疫法则定ＣＧＲＰ的释放。结果：咖啡因（１～１０ｍｍｏｌ／Ｌ）浓度依赖性地引起大鼠脊髓ＣＧＲＰ释放。无钙液不影响加入咖啡因０～１０ｍｉｎ时ＣＧＲＰ的释放，但减少１０～２０ｍｉｎ时的ＣＧＲＰ释放。钌红或Ｒｙａｎｏｄｉｎｅ预孵育可分别抑制咖啡因引起的ＣＧＲＰ释放。结论：咖啡因通过激活细胞内对Ｒｙａｎｏｄｉｎｅ敏感的钙池钙离子释放，从而直接引起大鼠脊髓释放ＣＧＲＰ。
Objective: To investigate whether caffeine can directly induce the release of calcitonin gene-related peptide (CGRP) from rat spinal cord and its mechanism. Methods: Spinal cord slices were incubated in vitro and the release of CGRP was determined by radioimmunoassay. Results: Caffeine (1 ~ 10mmol / L) induced CGRP release in rat spinal cord in a concentration - dependent manner. Calcium-free fluid did not affect the release of CGRP at 0-10 min with addition of caffeine, but reduced the release of CGRP at 10-20 min. Ruthenium red or Ryanodine preincubation inhibits Caffeine-induced CGRP release, respectively. Conclusion: Caffeine can release CGRP directly from the rat spinal cord by activating intracellular Ca (2+) release from Ryanodine-sensitive calcium cells.