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目前许多实验室采用麦康盖琼脂、去氧胆酸钠枸橼酸钠琼脂、沙门氏志贺氏琼脂等培养基,从污染标本中分离培养小肠结肠炎耶尔森氏菌(Yersinia enterocolitica,以下简称Ye)。但是Ye与变形杆菌等肠道细菌在上述平板上均为不发酵乳糖的小菌落,并且大多数肠道菌群生长较Ye快,故难以区别。本文报告了一种简单的选择性鉴别培养基(DYS培养基)。Ye在此平板培养基上能迅速生长,并与其他肠道细菌菌落有明显的区别,其成分及配制方法如下: 蛋白胨15克、酪蛋白水解物5克、牛胆汁粉末8.5克、脱氧胆酸钠20克、氯化钠5
Currently, many laboratories use Miconcon agar, sodium deoxycholate sodium citrate agar, and Schistosoma mansoni and other media to isolate and culture Yersinia enterocolitica (hereinafter, Yersinia enterocolitica) Referred to as Ye). However, intestinal bacteria, such as Ye and Proteus, are small colonies that do not ferment lactose on the plates, and most of the intestinal flora grows faster than Ye, making it difficult to distinguish them. This article reports a simple selective identification medium (DYS medium). Ye on this plate medium can grow rapidly, and with other intestinal bacteria colonies have obvious differences, its composition and preparation methods are as follows: peptone 15 grams, 5 grams of casein hydrolyzate, beef bile powder 8.5 grams, deoxycholic acid Sodium 20 grams, sodium chloride 5