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在完整的油菜植株上,利用喷叶法测定放线菌代谢物质对病毒病的治疗效果,以处理组的发病率此对照组减少的百分率表示疗效。从1958年到1962年先后测定6,376号次放线菌,找到不吸水灰色放线菌类羣(Actinomyces ahygro-scopicus Yen)的1695、3494、4084和4313四个菌株的代谢物质,在接种油菜花叶病毒(总6号)24小时后喷叶,有50%左右的治疗效果,接种前24小时喷叶有100%的防护效果。接种24小时后喷叶而未发病的植株叶内未能测出有侵染性病毒的存在。从喷药一次的效果来看,显然胜于0.5%2-硫尿嘧啶在油菜上的作用。1695、3494和4084三株放线菌发酵液的有效成分经15磅30分钟的处理后,在植株上测定效果,差异并不大。但是,所有有效浓度对植株均能引起不同程度的黄化等生理反应,使生长遭受一定程度的抑制,类似2-硫尿嘧啶所引起的生理反应。病毒与1695放线菌发酵液混合(1分钟),立即被钝化。发酵液中的有效成分能被叶细胞吸收,通过剪叶法及不同部位局部喷药及接种等初步证明,代榭物质昀有效成分运转的距离似乎极短。1695的发酵液在植株上的治疗效果常因接种与喷药相隔时间的长短而异。接种后3小时内喷的效果为100%,6小时降至90%,1—3天为25—45%,5—7天为5—10%。同时,在接种后3天内喷而发病的植株体内其病毒含量比对照少,3天以后喷的则此对照反而有增多的趋势。1695的发酵液在胶州白菜上对孤丁1号的治疗效果(接种后隔24小时喷)为92%,比崐山土种油菜上对总6号病毒的疗效高出三分之一。此种差异可能由于寄主植物的差异引起的。
In a complete rapeseed plant, the effect of the actinomycete metabolites on the viral disease was determined by the spray-on-leaf method, and the reduction in the percentage of the control group in terms of the incidence of treatment was reported as efficacy. From 1958 to 1962, 6,376 times of actinomycetes were determined, and 1695, 3494, 4084 and 4313 metabolites of non-water-absorbing Actinomyces ahygro-scopicus Yen were found. After inoculation of rape Leaf virus (total 6) sprayed leaves after 24 hours, about 50% of the therapeutic effect, spraying leaves 24 hours before vaccination has a protective effect of 100%. Inoculated 24 hours after spraying the leaves of the plant leaves failed to detect the presence of infectious virus. From the effect of spraying once, it is clear that the effect of 0.5% 2-thiouracil on rapeseed is obviously better than that of 0.5%. 1695, 3494 and 4084 three actinomycetes fermentation broth 15 pounds 30 minutes after treatment, the measured results on the plant, the difference is not significant. However, all the effective concentrations of phytochemicals induced different degrees of yellowing and other physiological responses, and inhibited the growth to a certain extent, similar to the physiological responses caused by 2-thiouracil. The virus was mixed with 1695 actinomycete broth (1 minute) and immediately passivated. The active ingredients in the fermentation broth can be absorbed by the leaf cells. It is initially proved that the active ingredients in the fermentation broth are operated at a very short distance by means of shearing the leaves and local spraying and inoculation of different parts. 1695 fermentation broth in the treatment of plants often due to inoculation and spraying the length of time varies. The effect of spraying within 3 hours after inoculation was 100%, reduced to 90% in 6 hours, 25-45% in 1-3 days and 5-10% in 5-7 days. At the same time, the virus content in the plants sprayed and sprayed within 3 days after inoculation was less than that in the control, but the control spray increased after 3 days. The treatment effect of 1695 fermentation broth on Gudou 1 on Jiaozhou cabbage was 92% (24 hours after inoculation), which is one third higher than the total number 6 virus on Kunshan soil rape. This difference may be due to differences in host plants.