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1977~1978年我们采用不同的保存方法,在三种温度下,以代表致病力强的和中等的两个菌株,进行了保存方法的比较试验.两年试验的结果如下:一、材料和方法1.培养基的种类和保存方法重蒸馏水:用重复蒸馏一次的蒸馏水,每管装5—7毫升.灭菌后将供试的新鲜菌种移入,成细菌悬浮液保存.0.1%蛋白胨水:1克蛋白胨加水1000毫升.处理方法同上.
From 1977 to 1978, we used different preservation methods to compare the preservation methods at three temperatures, representing the two strains with strong pathogenicity and moderate disease.The results of the two-year experiment are as follows: Method 1. The type of media and preservation methods Re-distilled water: repeated distilled once distilled water, each tube 5-7 ml. After sterilization will be tested fresh bacteria transferred into a bacterial suspension stored 0.1% peptone water : 1 g peptone plus water 1000 ml. Treatment as above.