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目的:观察神经生长因子(NGF)对体外培养大鼠雪旺细胞(SCs)增殖的影响。方法:采用MTT法观察NGF对SCs增殖的影响及对高糖引起的SCs增殖抑制的保护作用。结果:NGF能够促进SCs的增殖,同时对40 mmol·L-1葡萄糖(Glu)引起的细胞增殖抑制具有明显的改善作用,最佳浓度为100 ng·mL-1。结论:适量浓度的NGF可以促进SCs的增殖,同时对高糖引起的细胞增殖抑制具有明显的改善作用,从而促进周围神经损伤修复。
Objective: To observe the effect of nerve growth factor (NGF) on the proliferation of rat Schwann cells (SCs) cultured in vitro. Methods: The effect of NGF on the proliferation of SCs and the inhibition of proliferation of SCs induced by high glucose were observed by MTT assay. Results: NGF promoted the proliferation of SCs and significantly inhibited the cell proliferation induced by 40 mmol·L-1 glucose (Glu). The optimal concentration of NGF was 100 ng · mL-1. CONCLUSION: NGF can promote the proliferation of SCs and improve the inhibition of cell proliferation induced by high glucose, thus promoting the repair of peripheral nerve injury.