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[Objective] To study the Ilexgenin A-induced apoptosis in cervical cancer HeLa cells and its mechanism. [Methods] DAPI was adopted for nucleus stain to observe the morphological change during apoptosis; Apo-ONE Homogeneous protease -3/7 kit was used for measure the activity of Caspase -3/7; and the binding activity of nuclear extracts of cervical cancer HeLa cells NF-κB p65 with DNA was determined to investigate the activation level of NF-κB. [Results] DAPI assay showed that karyopyknosis and chromatin condensation occurred and apoptotic bodies appeared 24 h after Ilexgenin A (IA) induction. IA enhanced the activity of Caspase - 3/7 at the manner of dose-dependence. IA assumed remarkable inhibitory effect on the activation of subunit p65 of NF-κB, and this inhibitory effect was found dose-independent to some extent. [Conclusion] IA could remarkably induce the apoptosis in cervical cancer HeLa cells at the manner of concentration-dependence.
[Objective] To study the Ilexgenin A-induced apoptosis in cervical cancer HeLa cells and its mechanism. [Methods] DAPI was adopted for nucleus stain to observe the morphological change during apoptosis; Apo-ONE Homogeneous protease -3/7 kit was used for measure the activity of Caspase -3/7; and the binding activity of nuclear extract of cervical cancer HeLa cells NF-κB p65 with DNA was determined to investigate the activation level of NF-κB. [Results] DAPI assay showed that karyopyknosis and IA enhanced remarkable activity inhibitory effect on the activation of subunit p65 of NF-κB , and this inhibitory effect was found dose-independent to some extent. [Conclusion] IA could remarkably induce the apoptosis in cervical cancer HeLa cells at the manner of concentration-dependence.