论文部分内容阅读
对新建的克隆性人肾透明细胞癌细胞株ＲＬＣ－３１０进行了生物学鉴定和形态学观察。发现细胞可重叠生长，群体倍增时间３２ｈ，集落形成率４４％，具有超二倍体核型，染色体众数６４。细胞没有支原体污染，有特异性的ＬＤＨ同功酶谱，细胞接种Ｂａｌｂ／ｃ裸鼠可形成移植瘤。细胞大小均一，核大，细胞表面有丰富的微绒毛，未见到细胞间的桥粒结构。胞质内有变形的粗面内质网、线粒体和较多糖原颗粒，张力原纤维可见。细胞核异形性明显，核仁数目增多，有边集。表明这株细胞保持了肾透明细胞癌的基本特点。
The cloned human renal clear cell carcinoma cell line RLC-310 was identified and the morphological observation was performed. It was found that the cells could be overlapped and grew. The population doubling time was 32h, the colony formation rate was 44%, and the super diploid karyotype and chromosome number were 64. Mycoplasma without mycoplasma contamination, a specific LDH isoenzyme, cell inoculation Balb / c nude mice can form xenografts. Cell size is uniform, large nuclei, cell surface rich in microvilli, cell structure was not observed in desmosomes. The cytoplasm of the deformed rough surface endoplasmic reticulum, mitochondria and more glycogen particles, fibrillation visible. Significant nuclear heterotropism, an increase in the number of nucleolus, with edge set. Show that this strain of cells to maintain the basic characteristics of renal clear cell carcinoma.